A bioindicator is any species (an indicator species) or group of species whose function, population, or status can reveal the qualitative status of the environment. For example, copepods and other small water crustaceans that are present in many water bodies can be monitored for changes (biochemical, physiological, or behavioural) that may indicate a problem within their ecosystem. Bioindicators can tell us about the cumulative effects of different pollutants in the ecosystem and about how long a problem may have been present, which physical and chemical testing cannot.
A biological indicator is also the name given to a process for assessing the sterility of an environment through the use of resistant microorganism strains (eg. Bacillus or Geobacillus). Biological indicators can be described as the introduction of a highly resistant microorganisms to a given environment before sterilization, tests are conducted to measure the effectiveness of the sterilization processes. As biological indicators use highly resistant microorganisms, you can be assured that any sterilization process that renders them inactive will have also killed off more common, weaker pathogens.
The importance and relevance of biomonitors, rather than man-made equipment, is justified by the observation that the best indicator of the status of a species or system is itself. Bioindicators can reveal indirect biotic effects of pollutants when many physical or chemical measurements cannot. Through bioindicators, scientists need to observe only the single indicating species to check on the environment rather than monitor the whole community.
The presence or absence of certain plant or other vegetative life in an ecosystem can provide important clues about the health of the environment: environmental preservation. There are several types of plant biomonitors, including mosses, lichens, tree bark, bark pockets, tree rings, and leaves. Fungi too may be useful as indicators.
Changes in animal populations, whether increases or decreases, can indicate pollution. For example, if pollution causes depletion of a plant, animal species that depend on that plant will experience population decline. Conversely, overpopulation may be opportunistic growth of a species in response to loss of other species in an ecosystem. On the other hand, stress-induced sub-lethal effects can me manifested in animal physiology, morphology, and behaviour of individuals long before responses are expressed and observed at the population level. Such sub-lethal responses can be very useful as "early warning signals" to predict how population will further respond.
Amphibians, particularly anurans which consist of frogs and toads, are increasingly used as bioindicators of contaminant accumulation in pollution studies. Anurans absorb toxic chemicals through their skin and larval gill membranes and are sensitive to alterations in their environment. They have a poor ability to detoxify pesticides that are absorbed, inhaled, or ingested by eating contaminated food. This allows residues, especially of organochlorine pesticides, to accumulate in their systems. They also have permeable skin that can easily absorb toxic chemicals, making them a model organism for assessing the effects of environmental factors that may cause the declines of the amphibian population. These factors allow them to be used as bioindicator organisms to follow changes in their habitats and in ecotoxicological studies due to humans increasing demands on the environment.
Pond breeding anurans are especially sensitive to pollution because of their complex life cycles, which could consist of terrestrial and aquatic living. During the embryonic development of them, morphological and behavioral alterations are the effects most frequently cited in connection with chemical exposures. Effects of exposure may result in shorter body length, lower body mass and malformations of limbs or other organs. The slow development, late morphological change, and small metamorph size result in increased risk of mortality and exposure to predation.
Microbial Prospecting for oil and gas (MPOG) is often used to identify prospective areas for oil and gas occurrences. In many cases oil and gas is known to seep toward the surface as a hydrocarbon reservoir will usually leak or have leaked towards the surface through buoyancy forces overcoming sealing pressures. These hydrocarbons can alter the chemical and microbial occurrences found in the near surface soils or can be picked up directly. Techniques used for MPOG include DNA analysis, simple bug counts after culturing a soil sample in a hydrocarbon based medium or by looking at the consumption of hydrocarbon gases in a culture cell.
Automatic bioassay is possible, using the flagellate Euglena gracilis in a device which measures their motility at different dilutions of the possibly polluted water sample, to determine the EC50(the concentration of sample which affects 50 percent of organisms) and the G-value (lowest dilution factor at which no-significant toxic affect can be measured).
In Europe, a remote online biomonitoring system was designed in 2006. It is based on bivalve molluscs and the exchange of real time data between a remote intelligent device in the field (able to work for more than 1 year without in-situ human intervention) and a data centre designed to capture, process and distribute on the web information derived from the data. The technique relates bivalve behaviour, specifically shell gaping activity, to water quality changes. This technology has been successfully used for the assessment of coastal water quality in various countries (France, Spain, Norway, Russia, Svalbard (Ny Alesund) and New Caledonia).
The imposex phenomenon in the dog conch species of sea snail leads to the abnormal development of a penis in females, but does not cause sterility. Because of this, the species has been suggested as a good indicator of pollution with organic man-made tin compounds in Malaysian ports.